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21st September 2018

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Successful Development of a New Enzyme using Pichia Pastoris

fat and oil processingAPPLICATION: The customer required a bespoke lipase for use in an innovative new application for fat and oil processing in the food industry.

CUSTOMER CHALLENGE: To produce an enzyme capable of achieving the highly specific application needs identified by the customer.

APPROACH: Over the last 35 years Biocatalysts has developed significant expertise in developing and manufacturing enzymes using a range of hosts. This is an area that we continue to develop in order to ensure that our customers benefit from using the most cost effective and appropriate host for their specific enzyme application. In this project we used our expertise in Pichia pastoris to develop an effective basis for manufacture.

P.pastoris is one of Biocatalysts’ main expression systems for enzyme manufacture, in particular for enzymes to be used in the food industry. P.pastoris holds QPS (Qualified Presumption of Safety) status, which means it has undergone a safety assessment performed by the European Food Safety Authority and deemed safe for the production of food industry enzymes. In addition to this P.pastoris is effective at secreting over-expressed proteins at high yields whilst secreting a low volume of endogenous proteins and no endotoxins, leading to an enzyme product intrinsically ‘cleaner’ and less contaminated with unwanted proteins. As a yeast, P.pastoris also has the capability to produce more complex proteins than bacterial expression systems using their cellular machinery to perform complex protein folding and post-translational modifications.

Biocatalysts met the customers brief by identifying enzyme candidates for their process and assessing them against a suitable substrate. Once application trials proved positive and the best performing enzyme was chosen, the production was scaled up (applying “Design for Manufacture” principles) from small scale fermentation to large scale commercial production. Not only did the enzyme meet the precise specificity requirements, it has also been identified to be potentially useful in other non-food applications.

TIMESCALES:

Concept to small scale sample – 8-12 weeks
From small scale sample to large (multi Kilogram) sample – 12 weeks

BENEFITS TO CUSTOMER:

  • A bespoke enzyme meeting the customer’s specific requirements.
  • Access to Biocatalysts’ extensive expertise on expression hosts
  • Exclusivity for the customer’s application.
  • A reliable, routine and long term supply of enzyme.
  • Potential of using the same enzyme in other non-food applications.

Contact us to discuss the development, scale-up and routine manufacture of your enzyme.